Development of 27 novel cross-species microsatellite markers for the endangered Hucho bleekeri using

Abstract: In this study, we developed 27 polymorphic microsatellite loci in Hucho bleekeri, a glacial relict and freshwater resident salmonid fish in China. The number of alleles varied from 2 to 8 for each primer set. The observed and expected heterozygosity ranged from 0.250 to 0.906 and 0.508 to 0.845, respectively. The polymorphic information content ranged from 0.371 to 0.808. These microsatellite loci should be useful to study population genetics,paternity identification, speciation and adaptive evolution of this lineage.

Ke Wang, Shuhuan Zhang , Dengqiang Wang, Miaomiao Xin, Jinming Wu, Qingliang Sun, Hao Du, Chengyou Wang, Jun Huang, Qiwei Wei. Development of 27 novel cross-species microsatellite markers for the endangered Hucho bleekeri using next-generation sequencing technology. Conservation Genet Resour, 2015, 7: 263–267.

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