Isolation and characterization of eleven novel microsatellite loci of Brachymystax lenok tsinlingensis...

Abstract:Brachymystax lenok tsinlingensis is an endangered freshwater fish confined to cold-water mountain streams and rivers in Qinling Mountains of Shaanxi province, China. We isolated and characterized 11 microsatellite loci for Brachymystax lenok tsinlingensis and examined them for 42 individuals from Xushui River in Qinling Mountain. The number of alleles per locus ranged from 3 to 8, with an average of 5.3. The expected (He) and observed (Ho) heterozygosities were 0.3483–0.8874 and 0.4183–0.8421, respectively. Two loci deviated significantly from Hardy–Weinberg Equilibrium after Bonferroni correction. We expect these novel microsatellite markers from B. lenok tsinlingensis to be useful for population genetic conservation studies of the species.

附件下载:Haixia Liu, Yang Li, Xiaolin Liu, Guiwei Zou, Qiwei Wei. Isolation and characterization of eleven novel microsatellite loci of Brachymystax lenok tsinlingensis; a threatened fish endemic to Shaanxi, China. Conservation Genetics Resources. DOI 10.1007/s 12686-012-9810-7.pdf